FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell57.351000000956be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell57.351000000956be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261032
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT59800.024.7575382
CGATCTG25250.024.5935295
TCCGATC60150.024.5760733
CTTCCGA60400.024.572221
CCGATCT61050.024.1026734
CGATCTA9100.023.2412575
CGATCTT14800.021.2833215
CGATCTC18250.018.2461815
TAACGCC3200.016.8746344
TCGTTTA3100.016.69355630
TAAGGCG952.8213335E-416.5785885
ATAACGC3600.016.2500083
CGCATCG3350.016.11869813
AACGCCG3500.016.0710815
GCATTCG5600.015.66930322
CCGGTCG3450.015.65148920
ATTCGTA5800.015.128983524
GGCGTTA3150.014.99967642
ATCTGCG3300.014.9993447
GATCTGT9100.014.8348456
Produced by FastQC (version 0.11.2)