FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell56.35100000095695.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell56.35100000095695.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866780
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA18920.10135098940421475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG24450.021.1652285
TCCGATC53600.021.0723463
TTCCGAT54400.020.886542
CCGATCT54000.020.7912564
CTTCCGA54850.020.682471
CGATCTA7050.020.425135
CGATCTT12950.020.3277855
GACGTCG807.565814E-519.6876437
AGCGACG807.565814E-519.6876434
TAGCGAC600.00651171518.75013533
CGACGTC851.204886E-418.52954536
CGTCGCT851.204886E-418.52954539
ACCGTCG1902.3646862E-1117.762818
GTATTAA6100.017.3426931
CGCACCG800.001711304116.8751229
ATCTGCG2600.016.4419847
CGAACTA1004.1685515E-415.75011329
GCGACGT900.003754911515.00010735
TATAGAC3300.014.9997063
GTTATAG2601.0913936E-1114.7171631
Produced by FastQC (version 0.11.2)