FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell55.3510000009567b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell55.3510000009567b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130165
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9720.7467445165751162No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9060.6960396419928553No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.5831060576960012No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.5577536204048708No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.11984788537625322No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG1520.11677486267429801Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCCCAGGGAGACGTGTGCTCTTCCGATCTGCCCAGGGAGACGTGTGCTCTT1360.10448277186647717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCTG207.0105237E-444.99961531
CCCTAAC300.005130913629.9997442
CCCCTAT450.00121804525.0093941
GATCTTT20500.022.938836
ATCTTTT21000.022.1783817
TCTTTTT21900.021.2669418
CTTTTTT23000.020.2498289
CGATCTT25800.019.36035
CCCCCCT800.001699961816.87485544
GGGGTGC952.7955207E-416.57880616
TCTGACC850.00254895815.8822178
ACACATC1502.5314894E-614.99987145
CTCAGGG1055.986429E-414.9998712
GGTGCTC1108.5170264E-414.3180618
TCTAGCC950.0053425114.2104048
TCTGCCT950.0053425114.2104048
GGGTGCT1150.001191857913.69553517
CCCGGGA1656.7980473E-613.63624713
CCGGGGA1555.733523E-513.06440413
TACACAT1751.2472246E-512.85703244
Produced by FastQC (version 0.11.2)