FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell54.35100000095652.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell54.35100000095652.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035773
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA21840.10728111631306633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA7550.025.6277125
CGATCTG18500.024.6878155
TCCGATC45750.024.6871513
CTTCCGA46800.024.2924121
TTCCGAT46650.024.25912
CCGATCT46500.024.2405854
ACCGTCG2000.020.2483758
CGATCTT13300.019.1154795
TACCGTC2300.018.5854667
CGTCGTA2200.017.3849710
CGATCTC13200.017.0445065
ATCTGCG2700.016.665337
CGCATCG2301.8189894E-1216.629113
TTAACGG2201.2732926E-1116.36473735
CGGTACC2351.8189894E-1216.2752945
TCTTCCG23900.016.097435
ATAGGGG4950.015.9082043
ATACCGT2600.015.57605656
CCGTCGT2505.456968E-1215.2987739
AACCGTA900.003757251414.99879616
Produced by FastQC (version 0.11.2)