FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell53.3510000009562b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell53.3510000009562b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758490
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC40000.025.9854133
CGATCTG18900.025.9502985
TTCCGAT40500.025.8312592
CTTCCGA40500.025.7947791
CCGATCT40700.025.3173794
CGATCTT9900.023.1799585
CGATCTA5900.021.7355445
GTATTAG5500.018.0118731
CGATCTC11300.016.923425
GATCTGG11800.015.6343386
TTAACGG1451.8088867E-615.51820335
GTATTAT3050.015.5020211
CGTCGTA2202.1827873E-1015.33967910
GGCGTCG900.003752007815.00178232
AGGTCGC1654.2005195E-715.00092935
TACCGTC2559.094947E-1214.9987967
ACCGTCG2456.91216E-1114.69269858
CGCTAGA3400.014.5593137
ATAATAC7150.014.4743623
CCGTCGT2509.640644E-1114.3988449
Produced by FastQC (version 0.11.2)