FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell52.35100000095601.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell52.35100000095601.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672492
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA4450.028.3134985
TCCGATC33650.026.1431053
CTTCCGA34100.025.8896311
TTCCGAT34150.025.7603382
CCGATCT34550.025.2667374
CGATCTG15800.024.3503135
CCTAACG550.00393816920.45374320
CGATCTT9700.020.4115735
TGACGCG600.00651205818.74982537
CGCCTAT851.2046669E-418.52979334
CGATCTC9700.018.3240265
TACCGTC1802.0736479E-1017.4993137
TCTTCCG16150.016.8569245
TACGGGT850.002566490815.881734
GGCGTTA2151.546141E-1015.6970642
TATTCCG1156.787726E-515.651565
GTATTAG5150.015.3057621
CTATCGC1209.754666E-515.00030830
TGTAGGA2701.8189894E-1214.9994132
GTATTAA5000.014.8640821
Produced by FastQC (version 0.11.2)