FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell51.351000000955e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell51.351000000955e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054761
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC43050.024.5110423
CTTCCGA44150.024.1727961
CCGATCT43750.023.9645864
TTCCGAT44250.023.8971792
CGATCTA6550.023.3576815
CGATCTG19950.022.893665
CGATCTC11900.020.797345
CGATCTT10350.019.5642975
AACCGTA1403.092282E-919.2848116
ACCGTAC1403.092282E-919.2848117
TACCGTC2500.017.9991537
CCGTCGG2350.017.23449140
GTATTAG7100.016.8072531
CGCGAAG2450.016.5306422
CGCTAGA3950.016.51941737
CCCGTCG2650.016.13249439
TTACGCG850.00256689315.88160643
ACCGTCG2550.015.8816068
CGTCGTA2453.6379788E-1215.6115110
TCCGACG1301.1069649E-515.57732831
Produced by FastQC (version 0.11.2)