FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell50.351000000955cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell50.351000000955cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368291
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA23410.17108933699045015No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC14160.10348675829922145No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG13960.10202508092211379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA4250.025.9400165
CTTCCGA28400.025.7706321
TCCGATC30100.024.4424293
TTCCGAT30050.024.4082262
CCGATCT30300.024.206844
CGATCTG14350.023.9884765
CGCTATA702.703649E-522.49981739
TCTTCCG13450.020.5752895
TACGCAG550.003937934520.4536325
TCCGAAC807.564812E-519.68734244
GCGGAAT807.566937E-519.6866219
CGATCTT7350.019.5909615
ACCGTCG1850.019.458598
GCTCTTC14300.019.3522833
CCGGGAT951.3166382E-518.94652214
TGTCCGA600.00651279418.74916310
CGATCTC7400.018.2424285
AATCGGG1507.2686817E-917.99985523
CGAACTA1406.476512E-817.67907329
TACATCG901.8666421E-417.4992185
Produced by FastQC (version 0.11.2)