FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell5.35100000095021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell5.35100000095021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991117
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA26580.13349290875423192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA41300.029.4371851
TCCGATC42150.028.7179623
CGATCTG18550.028.5024365
CCGATCT42800.028.2811164
TTCCGAT43550.027.846432
CGATCTA6300.025.7128375
CGATCTT10700.024.3911485
TACGCAA903.291243E-722.49873210
TTCGCGA500.0022659322.49873244
TCTTCCG16000.022.0768815
CGATCTC12250.021.8559115
CGATTAC754.606806E-520.99881745
ACGGGTA1009.066662E-720.248866
GCTCTTC17550.020.1275753
GCGGAAT1351.9681465E-919.99887319
GAATCGG1850.019.4588522
GATCTCG5700.019.3410156
CGTAGCG1454.782123E-918.6196415
CCCAACG1102.2588847E-618.40805221
GTGCTCT19650.017.8737071
Produced by FastQC (version 0.11.2)