FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell49.351000000955a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell49.351000000955a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392598
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA20220.14519624471670936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGA300.00514859229.99885238
CGATCTG17450.025.7879035
CTTCCGA38800.025.3578241
TTCCGAT39050.025.121582
TCCGATC39050.025.0639613
CCGATCT39350.024.8156954
CGATCTA5200.024.2307115
CGATCTT9450.023.8094655
CCGGGTA1703.6379788E-1219.8521814
GGCGTTA1901.8189894E-1218.94664242
CGGCGTT2251.8189894E-1216.9993541
CGATCTC10700.016.8223925
GCGTTAT2450.016.5299843
CGGGTAT1104.64017E-516.36300815
GTATTAG6100.015.8708751
GATCTGG11550.015.7791836
TCTAGAC2051.3169483E-915.3658173
CGTTATT2353.6379788E-1115.31856244
GATCTGC8750.015.17139156
TCTTCCG18250.015.1643475
Produced by FastQC (version 0.11.2)