FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell48.35100000095589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell48.35100000095589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973229
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA23970.12147601722861362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG28500.020.9203935
TCGGTTA550.003937086620.45493737
CGGTCGA908.262041E-619.99835620
CGATCTA11100.019.8642395
CCGATCT67500.019.7660434
TCCGATC67800.019.7449533
TTCCGAT68950.019.415632
CTTCCGA69000.019.3530221
ATCGCGG1051.447781E-619.28412816
CGGACTA951.3158198E-518.94869231
CGATCTT12750.018.8817585
TACCGTC3200.017.576687
CGTCGTA2950.017.54093210
ATCCGTA901.867706E-417.49856212
CGGTACC2200.017.38537445
CATCGCG1053.1164498E-517.14144715
TTAACGG2301.8189894E-1216.63159635
CCGTTAA2057.4578566E-1116.4620616
TCGCGGT1104.643493E-516.36229117
ACCGTCG3200.016.1705468
Produced by FastQC (version 0.11.2)