FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell47.3510000009556f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell47.3510000009556f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419466
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT59100.022.841822
TCCGATC59450.022.7073443
CTTCCGA59850.022.683831
CGATCTG27500.022.6628255
CGATCTA9700.022.4991955
CCGATCT60050.022.4804614
CGATCTT12750.019.411075
GCGTAAT706.956199E-419.2981851
GTATATA8050.016.5013471
CGATCTC17450.015.3432895
CCCCTAT7850.014.9140961
GTCTATA2509.640644E-1114.409311
TTAACGG2355.875336E-1014.36207835
CTATACC9400.014.1218354
TATACCT10150.013.9650165
CGAACGA1453.1088E-513.96472916
TAATACC4200.013.9280724
GTATTAG8100.013.8978691
GCATTCG5050.013.81167222
ACACCGT2652.4374458E-1013.5844196
Produced by FastQC (version 0.11.2)