FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell46.35100000095546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell46.35100000095546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214187
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA26730.12072151087509772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA10150.024.8275995
CTTCCGA59450.024.7352851
TCCGATC59300.024.7006863
TTCCGAT59700.024.6105652
CCGATCT59800.024.5317844
CGATCTG24400.023.6987845
CGACCGT500.00226555822.49950419
CGATCTT14400.020.7812615
CGATCTC16850.020.0296845
AGCCGTA600.006513186718.74958611
TTAACGG2351.8189894E-1216.27623735
GATCTAC5450.015.6880826
GATCTAG5350.015.5607556
TCGCGCT900.003755936614.99966923
TCTTCCG29150.014.8199045
GGCGTTA2900.014.74105342
GTATTAG8450.014.6575571
CTATACC13300.014.548884
GATCTAA4650.014.5161366
ATTACGG950.00537807714.2105335
Produced by FastQC (version 0.11.2)