FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell45.3510000009552c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell45.3510000009552c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530025
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT33920.13406982144445215No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTTG33370.1318959298821158No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGTA31660.12513710338830644No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCG27120.1071926166737483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG29050.023.3895155
CCGATCT73100.021.7293994
TCCGATC73400.021.6716673
CTTCCGA74300.021.5110571
TTCCGAT77850.020.490692
CGATCTA13000.020.2489685
CGATCTT17900.019.8593245
TGCGCGG807.570547E-519.68649910
GTATTAG7700.018.1256311
GTATATA11650.017.9700471
AAGTTCG8500.017.73579236
CGGTCAA8400.017.40982618
AGTTCGC8550.016.84224537
CGGTACT2159.094947E-1216.74432638
GATCTGT9900.016.5900636
ATACGTC3450.016.30351620
AGCCGGT8700.016.29227415
GTTCGCT9000.016.00013438
GTACTAA2550.015.8900231
ACGTCCG850.00256650315.88217143
Produced by FastQC (version 0.11.2)