FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell44.35100000095502.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell44.35100000095502.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999328
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA23800.11903999743913954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG22400.021.1936325
TCCGATC52550.021.1079043
TTCCGAT52600.021.0450652
CCGATCT52850.021.0306594
CTTCCGA52800.021.0242611
CGATCTT12600.020.89235
CGCCGTA654.239789E-420.76919625
CGATCTA8500.019.3230135
GTATTAG7550.017.2977181
CTCGGTA3250.015.92424627
CGGTACC2900.015.5164445
GTATTAA5950.015.5158181
TTAACGG2950.015.25421235
TATTAGA5050.015.1481112
GCGTTAT2253.0559022E-1014.99922443
CCGATCG4550.014.83439814
CGATCGC4750.014.68345115
GGCGTTA1859.629912E-814.5938442
CGATTAC1402.232684E-514.46353845
CGATCTC15150.014.4055575
Produced by FastQC (version 0.11.2)