FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell43.351000000954e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell43.351000000954e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481210
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA26270.1058757622289125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGT352.8213358E-432.14281536
TCCGATC69600.022.6934783
CCGATCT70300.022.3714964
CTTCCGA71750.022.0599371
CGATCTG27200.021.9204835
TTCCGAT72300.021.8771252
CGATCTA10350.021.7386635
TACGTCC654.240754E-420.76878445
ATACCCG550.00393829520.45410544
CGATCTT18250.019.9721725
TCGACAC2850.018.946963
ATTCGAC2650.018.691651
CGATCTC21200.018.5727165
GTATTAA8000.018.2933921
CGGTAAG800.001711614916.87497928
CGGTCAA5350.016.8220718
GCATAGA1552.0532934E-715.9783471
GTATTAG6150.015.7421551
AGCCGGT6050.015.61949915
CTCGTTA2900.015.51690811
Produced by FastQC (version 0.11.2)