FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell42.351000000954cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell42.351000000954cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2119064
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG19850.023.689375
TTCCGAT47550.023.65852
TCCGATC47650.023.561633
CCGATCT48450.023.1725864
CTTCCGA48800.023.0205121
CGATCTT13250.019.8672475
CGATCTA7350.019.591175
CGGTCGA951.31734305E-518.94627420
CGATCTC13750.018.653915
GTATTAG7750.018.3009261
TTAACGG2005.0931703E-1116.8756235
GCATTCG4200.016.0708822
CGGTACC2151.546141E-1015.6967745
TCTTCCG27050.015.4708225
GCTCTTC27350.015.0543313
ATCTGCG2405.0931703E-1114.9991367
ACCGTCC1351.583666E-514.9991358
TATTAGG4700.014.8399192
GTATAAC2801.8189894E-1214.47267151
CATCGCG1402.2329366E-514.46345115
Produced by FastQC (version 0.11.2)