FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell41.351000000954a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell41.351000000954a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005108
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24190.12064188063685348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGA501.9829786E-631.49921438
CGATCTG20450.026.5158925
TCCGATC47300.026.210363
TTCCGAT47600.026.0451682
CTTCCGA48100.025.9335161
CCGATCT47900.025.78814
CGATCTA6950.025.2517995
CGATCTT11950.021.4644365
TATACCG855.0352955E-621.176476
TCGAGTA754.6057154E-520.99947541
CGGGTAT654.241175E-420.76819415
CGATCTC13550.019.7601495
ACAATCG807.568034E-519.6870137
GTATACG600.006512148318.7499982
GCGTAAG600.006513085718.7495323
GATCTGG13650.017.1428576
CGCCGTA1053.114959E-517.1424325
ATCTACG1501.4250872E-716.4991767
GTATTAG6450.016.4072131
TCTACGC1104.6422665E-516.362828
Produced by FastQC (version 0.11.2)