FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell40.3510000009548a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell40.3510000009548a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179987
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24120.11064286163174368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5800.028.3175665
CGATCTG18300.025.3266185
CTTCCGA43900.024.9248031
TCCGATC43900.024.9076523
TTCCGAT43900.024.8564032
CCGATCT45200.024.1912824
CGATAAG550.003938257720.45400243
CGATCTT12400.018.6885915
GTATATA8350.017.7957971
GGCGTTA2400.016.87455242
CGATCTC14300.016.6774985
AATCGTC952.819407E-416.58002928
TTAACGG1904.4929038E-1016.57926835
GTATTAG7800.015.8755241
TCTTCCG21850.014.82764155
CCCCTAT10800.014.8011211
CGGTACC2457.094059E-1114.693150545
GTATAAA6150.014.6434981
CTATACC12300.014.6334234
TAATACG1705.897946E-714.5581044
Produced by FastQC (version 0.11.2)