FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell4.35100000095014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell4.35100000095014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859442
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27350.1470871368937563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5250.033.4276125
CGATCTG20350.030.4045315
CTTCCGA43100.029.6172371
TTCCGAT44000.028.8912182
TCCGATC44200.028.7604873
CCGATCT44700.028.3884474
CGATCTC12150.022.4067635
TCTTCCG16800.022.097585
GATCTGC9750.021.2301626
ATCTGCG2550.020.2935357
GCTCTTC18750.019.5594393
GATCTCG5700.018.9468256
CGGGTAT951.3168719E-518.94682315
GTATACG600.006489833418.7610651
CCGTCGA600.00651204318.74996641
GTGCTCT19700.018.3991781
GTCTATA2250.018.0106221
CGATCTT11500.017.9994835
TATCCGA800.001711094216.87542329
AACGCCG2403.6379788E-1215.9370435
Produced by FastQC (version 0.11.2)