FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell39.35100000095460.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell39.35100000095460.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949672
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG27200.024.1533575
CTTCCGA65100.023.6579691
TCCGATC64700.023.6121963
CCGATCT65150.023.5177754
TTCCGAT65700.023.2185572
CGATCTT17450.021.5320115
CCGTCGA1156.7811925E-921.52152641
GTCGACG1201.10740075E-820.62444943
CGATCTA9450.020.4752965
CGTCGAC1251.7706043E-819.7994742
TCGACGG1354.2809916E-818.33253144
TACGCAA1406.4894266E-817.677810
GTATTAA9150.017.4718781
CGATCTC18450.017.0724245
ATAGGGG5600.016.8745483
GGCGTTA3000.015.7495842
GGGGTTA4450.015.6734726
TAACGCC3450.015.651494
CTTACCG1156.790799E-515.651499
CGTACGT900.003756023514.99985341
Produced by FastQC (version 0.11.2)