FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell38.35100000095447.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell38.35100000095447.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311808
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA8650.022.8890295
TCCGATC62850.022.4809763
CCGATCT63150.022.3741784
TTCCGAT63650.022.1984182
CTTCCGA64200.022.0941051
CGATCTT15800.021.7868865
CGATCTG25500.021.5283365
CGATCTC18850.017.0681115
GTATTAC3600.016.2607941
GGCGTTA2850.015.78936942
TACACCG2051.3205863E-915.3650865
GTATTAG8050.015.1031921
TATTAGA7950.014.7162462
TTAACGG2601.0913936E-1114.71207535
GTATTAT4600.014.6836591
GTCTATA3250.014.5481191
CGCGAAG3000.014.24959622
GATCTGG17550.014.1018586
CCCCTAT9950.014.0294131
GCGTTAT3400.013.89666543
Produced by FastQC (version 0.11.2)