FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell37.3510000009542d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell37.3510000009542d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319974
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA57520.17325436885951517No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG33350.10045259390585588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG35800.031.0468755
CTTCCGA72850.030.4734941
TCCGATC73150.030.2967363
TTCCGAT73650.030.0910572
CGATCTA8900.029.3252645
CCGATCT75550.029.304524
CGATCTT18000.023.8745275
ATCTGCG5950.021.1757347
CGATCTC21000.020.1424585
TACGCAG951.3172792E-518.9469935
CGGTAAG600.006512636318.75019528
TATAGCG600.006513769318.749635
GATCTGG26650.018.7425966
TCTTCCG28100.018.4160065
GATCTAC6500.016.9612056
GATCTGC18950.016.7410936
GGTACTA6650.016.57911939
CGTTATA1104.642544E-516.3633142
TTGTCGC7950.016.13224237
GTATTAA7150.016.0597611
Produced by FastQC (version 0.11.2)