FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell36.35100000095403.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell36.35100000095403.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933085
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA37630.12829495224311604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC352.8217514E-432.142253
CGATCTG31050.029.4921955
CTTCCGA62850.028.4799881
TTCCGAT63050.028.4054892
TCCGATC63200.028.2668693
CGATCTA9150.027.540465
CCGATCT65100.027.338194
GTCGACG754.6064342E-520.99960143
CGATCTT15550.020.8356195
ATCTGCG4300.020.4062447
CCGTCGA807.5692114E-519.68712641
TCGACGG951.3171844E-518.9470144
TCTTCCG28700.018.7365785
CGATCTC15750.018.4282235
GCTCTTC29750.018.2265453
GTGCTCT30100.018.1768111
GGCGTTA5000.017.54966742
CGTCGAC1053.1155927E-517.14253242
GATCTGC16900.017.0410986
GATCTAG7100.016.7954566
Produced by FastQC (version 0.11.2)