FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell35.351000000953f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell35.351000000953f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713847
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27510.1013690160130619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC77250.023.970623
CCGATCT78150.023.6657754
CTTCCGA79300.023.6232811
TTCCGAT79250.023.5360262
CGATCTT19200.022.9685065
CGATCTG32200.022.8491345
CGATCTA10200.022.7203465
CGGGTAT1551.8189894E-1221.7735615
TTAACGG2700.020.83349635
CGATCTC23300.019.1199675
CGAACTA2450.018.36748929
GTATTAG10100.016.0509661
CGGTACC3650.016.02693245
TACGGGT850.00256655415.8821834
GATCTCG9200.015.4074446
TGCCGGG4000.015.18705912
CGCATCG4150.015.18028213
GTATATA10250.015.1570721
GTAGCGT900.003755940614.99984125
TAACGCC4200.014.999844
Produced by FastQC (version 0.11.2)