FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell34.351000000953ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell34.351000000953ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111259
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA9100.024.2306945
GGCGTTA2250.022.99992642
TCCGATC62600.022.8593523
CTTCCGA63250.022.7807541
TTCCGAT63600.022.499932
CCGATCT63400.022.3934634
CGATCTG24250.021.8040565
CGATCTT18550.020.0134145
GCGTTAT2850.018.94730843
CTAGCGC600.00651237918.749944
CGATCTC17650.017.8469685
CGTTATT2800.016.87414744
TATATCG952.8208242E-416.5788965
GTATTAG8150.015.7458421
TCTTCCG34100.015.04394155
AATACGC900.003755386514.9999535
CGTCGTA2101.8553692E-914.99924210
ATCTGCG2751.8189894E-1214.7265297
GATCTCG6900.014.6738666
CCCTAGT2152.562956E-914.6601451
Produced by FastQC (version 0.11.2)