FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell33.351000000953a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell33.351000000953a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142216
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10880.7650334702143219No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9670.6799516228835012No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8660.6089328908139731No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8390.589947685211228No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT2300.16172582550486583Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG2200.1546942678742195Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCCCAGGGAGACGTGTGCTCTTCCGATCTGCCCAGGGAGACGTGTGCTCTT1720.12094279124711706Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG654.8243055E-727.69144435
TTCTAGA507.048716E-527.0371821
CGATCCT651.5119525E-524.23001334
ATCCTGT751.6807262E-623.9992536
CCGATCC702.6821885E-522.49929633
TCCTGTC1302.7226633E-819.03786837
CTTATAC3150.017.1423245
ATCTTTT16400.016.7372827
GATCTTT16700.016.7060646
TCTTATA3400.016.543644
TCTTTTT16950.016.1941838
CTCTTAT3500.016.07092743
TCCTGGG1004.1360984E-415.7495082
TCTCTTA3650.015.41047742
CTTTTTT18250.014.9173429
CGATCTT21750.014.3788615
TCTGCGC1108.525339E-414.3177348
CTAGACG1801.102926E-613.749573
GTCTCTT3950.013.67045941
TGTCTCT4200.012.85674140
Produced by FastQC (version 0.11.2)