FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell32.3510000009538b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell32.3510000009538b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148732
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7400.4975391980206008No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.4040825108248393No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.370464997445069No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.3173493263050319No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT2440.1640534652932792Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG2200.14791705887098944Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTGCAGGGAGACGTGTGCTCTTCCGATCTGTGCAGGGAGACGTGTGCTCTT1520.1021972406745018Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCC207.015018E-444.99798227
CTCGCAA250.002101688935.99838622
TCGCAAG300.00513415229.99865323
TCGCTAC300.00513415229.99865327
CTTGGGG550.00392170620.4536292
AAGCTGT550.00392170620.45362936
AACAGAT600.006486169518.7491597
CTGCCAA750.001104388317.99919328
ATCTTTT14200.017.4287957
GATCTTT14300.017.3069176
TGCCAAG800.001701916316.87424329
TACTGGG1104.595777E-516.3629042
TCTTTTT15050.016.294958
TCCCTAA850.00251768315.9137471
CCTGCAC850.002551870715.88164132
CTTTTTT15850.015.4724929
CCTTGGG900.003734604214.9993272
GCACAGA1402.2052054E-514.4636386
CGATCTT20400.014.22730355
GCCGGGG950.00534854314.20988941
Produced by FastQC (version 0.11.2)