FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell31.35100000095361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell31.35100000095361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012520
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25060.12452050165961083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA6900.022.498885
TTCCGAT49200.022.3616942
TCCGATC49250.022.3389913
CTTCCGA49600.022.1995581
CCGATCT49500.022.089814
CGATCTG20450.021.8937785
CGATCTT12900.020.2315525
GCCGTTA1803.916284E-916.24919315
CGATCTC14450.015.8815635
CGGTACT2700.015.83333338
TAACGCC2009.295036E-1015.7492184
CTAGCCG1004.1705545E-415.7492189
GTATTAG7750.014.8178641
GAACCGT1553.6222773E-614.5154086
CCGTTAA2052.1818778E-814.26758416
GGTACTA3000.014.2539
ATCTGCG2853.6379788E-1214.2098217
ATAACGC2359.038558E-913.403593
GCGTTAT1851.5297046E-613.37804643
TTGCGGT3550.013.31018935
Produced by FastQC (version 0.11.2)