FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell30.35100000095348.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell30.35100000095348.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432365
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA8850.024.4051195
TCCGATC57200.023.9931223
CTTCCGA57750.023.8991781
CCGATCT57200.023.8751244
CGATCTT16500.023.7256575
TTCCGAT58100.023.6989022
CGATCTG21550.023.490285
TATAACG850.00256743515.8812712
TCTTCCG29500.015.8633275
CGGTCGA1156.791431E-515.65110720
CGATCTC16700.015.3582355
GGCGTTA2405.0931703E-1114.99928742
TACCGTC3150.014.9986717
CGTCGTA2900.014.74007310
TAGGGGT4150.014.6375584
GTGCTCT33150.014.3988171
GTATAAA5850.014.2403391
CGGGTAT950.00538145814.20926815
ATAGGGG4300.014.12694553
GCATTCG5500.013.90871522
Produced by FastQC (version 0.11.2)