FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell3.35100000094ffc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell3.35100000094ffc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171292
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA17780.15179818525184155No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGG12770.10902490583048463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA2500.029.6980845
CTTCCGA22800.028.044861
TCCGATC23050.027.427733
CGATCTG9000.026.9982595
CCGATCT23500.026.9025214
TTCCGAT23650.026.8270232
TACCGAA450.001227030524.99838611
GGTACCT8450.022.6316769
CGATCTC7250.021.412415
GCCAGTA9150.020.90296726
TCTCGTA550.00393805420.4532242
ATCTGCG1109.991345E-820.4532247
CGATCTT8100.020.276475
TCTTCCG10300.020.095795
GTTGATC9900.019.9987117
TACCTGG9800.019.74362611
ACCTGGT9850.019.64340212
CATATGC9850.019.41747734
GGGTACC9900.019.3169368
GATCCTG9950.019.21986420
Produced by FastQC (version 0.11.2)