FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell29.3510000009532e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell29.3510000009532e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983854
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA28490.14360935834995922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT44050.024.4647732
TCCGATC44200.024.4326523
CTTCCGA44450.024.3605841
CGATCTG19700.023.8688625
CCGATCT45700.023.5814724
ACGTCCG1003.648529E-822.49953343
CGATCTT12150.020.9244385
CGGTCGA654.240318E-420.768820
CGATCTA6650.019.9609855
CGATCTC13150.017.622325
GTCTTAT2500.017.1091331
AGACCGT1459.685755E-817.067756
GGGGTTA4950.016.8169866
CGCTTAG850.00255692115.8908351
TAGGGGT5950.015.8812234
TTAACGG2302.5465852E-1115.65303335
GACCGTC1301.1079455E-515.5758157
TCAACGC2900.015.51809325
GTCCTAT6100.015.1310281
TCTTCCG22700.015.0650085
Produced by FastQC (version 0.11.2)