FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell28.35100000095304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell28.35100000095304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208817
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG18000.026.7488425
TCCGATC44450.026.1181053
CTTCCGA44850.025.9500181
TTCCGAT44950.025.8776322
CGATCTA7100.025.6679045
CCGATCT45700.025.4529464
CGATCTT13300.020.9765385
GGCGTTA3000.017.24964542
CGATCTC12950.017.026295
ATACCGT3150.016.427866
GTATTAG6600.016.3721941
GTATTAA6050.016.3721941
TACCGTC2900.016.2923987
TCTTCCG22100.015.6780555
GATCTAG4000.015.1868436
CGAGCCG4600.015.16238915
GTATAGA4450.014.6705891
TAACGCC2355.875336E-1014.3610814
GTGCTCT24600.014.2757541
TAGCGAC950.00537640114.21119933
Produced by FastQC (version 0.11.2)