FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell27.351000000952e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell27.351000000952e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526137
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA33290.13178224300582272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA8700.027.154545
TCCGATC56250.025.75943
TTCCGAT56500.025.6055982
CTTCCGA56400.025.5914731
CGATCTG23600.025.4549145
CCGATCT57500.025.0428944
ATCTGCG2650.020.376487
CGATCTT16950.020.1765215
CGATCTC14750.018.7622745
ACACCGT2550.018.528986
TCTTCCG25300.017.3414925
GATCTAG5650.015.5306116
CGATTAC1754.8734364E-815.42790745
CGGTACC2950.015.2535845
CCCGCGA3600.014.9996520
GCTCTTC29550.014.9235113
GCATTCG5150.014.85402422
CGCTAGA5050.014.70291937
TTTATCG2702.1827873E-1114.16633740
CCGCTAG5250.014.14308736
Produced by FastQC (version 0.11.2)