FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell26.351000000952cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell26.351000000952cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103110
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27640.13142441431974553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA6750.027.6657455
CTTCCGA45750.025.147051
TTCCGAT45850.025.026432
TCCGATC46050.024.9177383
CCGATCT46700.024.474564
CGATCTG20100.023.6186185
CGACGTA500.002265355322.49978626
TTAACGG2800.020.09005235
CGATCTT12900.019.883065
GTATTAG7650.018.541141
CGGTACC2950.018.30447445
CGATCTC12900.017.7901065
CGTCGTA2750.017.18124810
ACCGTCG2950.016.77918
ATACCGT2950.016.77916
TACCGTC3100.016.6929937
TAACGCC2101.07320375E-1016.0708944
CTCGTTA2950.016.01641511
TATGCGC850.002565462815.88295729
GTCTAAT2009.222276E-1015.7599681
Produced by FastQC (version 0.11.2)