FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell25.351000000952a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell25.351000000952a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071864
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24740.11940938208299386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG21250.028.5869465
CTTCCGA50150.027.2544351
CGATCTA7150.027.0617165
TCCGATC51350.026.551873
CCGATCT51850.026.2524324
TTCCGAT52800.025.8653132
CGATCTT13600.021.0100825
TCTTCCG23000.019.173055
CGATCTC14650.019.043515
CGCGGAT600.006513006518.74960742
GCTCTTC24250.018.2775273
GTATATA8050.017.6223621
CGAAAAC4600.017.60875123
GATCTAG3600.017.4992126
CGGTCGA1305.7908437E-717.30691320
GATCTGT6400.017.2257866
AACCGCG4600.017.11920741
ACAACGC1205.1757925E-616.87546233
CGATCCT800.001711157616.87546228
CGGACGT800.001711986716.8742444
Produced by FastQC (version 0.11.2)