FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell24.3510000009528c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell24.3510000009528c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6280
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC150.23885350318471338No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC120.1910828025477707TruSeq Adapter, Index 1 (95% over 23bp)
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTT100.15923566878980894No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGA80.12738853503184713TruSeq Adapter, Index 1 (96% over 29bp)
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTA70.11146496815286625No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)