FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell23.35100000095262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell23.35100000095262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648226
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA39120.1477215313194569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA9600.025.0769045
TCCGATC63900.023.1326793
CTTCCGA64300.022.8993641
TTCCGAT64700.022.8466472
CCGATCT64800.022.776674
CGGGTAT1500.022.49890515
CGATCTT16100.022.079675
CGATCTG25650.021.0516075
CGATCTC19950.017.3675775
GTATATA12100.016.373971
TCGTCCG1257.637225E-616.19921110
CGATTAC1552.0697007E-715.96726645
GGGGTTA6400.015.8195436
CTCGTTA2950.015.25349411
TTAACGG3550.015.21196435
TAGGGGT6950.014.8913624
GTATTAG9550.014.6165531
GATCTAA5400.014.5826246
ATACCGC4200.014.46494827
ATAGGGG8250.014.4538423
Produced by FastQC (version 0.11.2)