FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell22.35100000095256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell22.35100000095256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135891
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA32790.1535190700274499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG28750.023.4774915
CCGATCT72000.021.5930424
TTCCGAT72050.021.5156022
TCCGATC72000.021.4992943
CGATCTT17400.021.3355085
CTTCCGA73300.021.2284681
CGATCTA11350.019.6249085
CGGGTAT1651.2332748E-917.72669215
GATCTGT12150.016.8513016
CGATTAC1652.3623215E-816.363145
CGGTACT3350.016.11925338
GGTACTA3500.016.07127839
CGATCTC18450.015.9750865
TTGCGGT3600.015.62521935
TGCCGGG3050.014.75361412
GTATTAG7800.014.7238091
CCGGGTA1705.8966725E-714.55834714
GGGGTTA5600.014.4638116
CCTTGCG3750.014.40020233
ACCGTAT1108.594727E-414.3177138
Produced by FastQC (version 0.11.2)