FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell21.3510000009522f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell21.3510000009522f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228275
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA9400.023.935685
TCCGATC60550.023.595723
CTTCCGA61000.023.5123041
TTCCGAT60800.023.4986972
CCGATCT61200.023.3818764
CGATCTG25100.023.2166585
TCGAGTA802.9767816E-622.50004641
ACAATCG855.035625E-621.17651237
CGATCTT15050.020.481315
CAATCGA1051.446786E-619.28575338
CGATCTC17700.018.6860585
CGCTATC1053.115689E-517.14212212
CGCTAAC952.8212855E-416.57860844
GTATTAG8100.016.1223581
CGGGTAT1602.968045E-715.46808715
GATCTAG5200.015.1439216
ATCGCTA1209.762262E-514.99935510
ATCTCGT3200.014.7649927
ATCTGCG2751.8189894E-1214.7266417
CGCGCAA2456.91216E-1114.69357721
Produced by FastQC (version 0.11.2)