FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell20.35100000095205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell20.35100000095205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723945
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA17720.10278750192146502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAC755.6439603E-826.99933842
GTCGACG755.6455974E-826.99855643
CGATCTA5300.026.3193455
TCCGATC42300.025.849683
CTTCCGA43100.025.4935021
TTCCGAT42900.025.4881462
CCGATCT43250.025.3339044
CCGTCGA801.0543772E-725.31261441
CGATCTG18850.024.9456145
TCGACGG955.537895E-721.3146544
CGATCTT11800.021.1641225
CGATCTC11600.021.1411115
CGACGGC1302.7763235E-819.03744345
TACCGTC2050.018.657547
ATCTGCG2550.018.528427
CCGTTAA1406.485425E-817.67762616
CGTCGTA1802.0736479E-1017.49906310
GCCGTTA1551.0917574E-817.41842315
GGCGTTA2301.8189894E-1216.63002642
ACCGTCG1904.4929038E-1016.578068
Produced by FastQC (version 0.11.2)