FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell2.35100000094fd2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell2.35100000094fd2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002664
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA15680.15638339463668788No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGG11760.1172875459775159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG300.005147339329.9995375
CGATCTG7750.027.8705355
CTTCCGA20950.027.0829261
TCCGATC20900.027.0211123
TTCCGAT21400.026.6000542
CCGATCT21550.026.206094
GGCGCTA450.001226556324.9996136
CGATCTA2900.023.27555
GCGCTAA500.00226461422.4996517
CGATCTT7250.021.413465
TCTTCCG9600.021.3277955
GGTACCT7050.020.744369
ATCTGCG1552.3646862E-1120.3222667
CGGTACC1251.7669663E-819.79969445
CGATCTC6850.019.3792615
GTGCTCT11000.019.0357231
GCATTCG951.3157338E-518.94707522
CCGCGCT600.00651051318.7497130
GCTCTTC10700.018.7146633
ACGCTTC1102.2557306E-618.40880421
Produced by FastQC (version 0.11.2)