FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell19.351000000951ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell19.351000000951ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908525
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19930.10442619300244954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG20100.025.1858025
TCCGATC48850.022.7065813
TTCCGAT48750.022.660852
CGGACGT500.002265552322.4993236
CTTCCGA49400.022.430561
CCGATCT49400.022.3626824
CGATCTA7100.022.1824265
TTAACGG1800.021.24935735
CGTCTAA550.003938166420.45392640
CGATCTT13450.019.2373355
CGGTACC2250.017.99945445
GTATTAG7050.016.9312381
GTATTAA6850.016.768011
TCGAATT1257.633653E-616.19950940
ACCGCCT2550.015.88187212
ATCTGCG2550.015.8818727
CGATTAC850.002566516615.88187145
ATATGCG900.003755886814.9995464
AAACGTC1351.5830912E-514.99954640
GATCTGG12650.014.940266
Produced by FastQC (version 0.11.2)