FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell18.351000000951c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell18.351000000951c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178463
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25360.1164123512770242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA8300.024.6673665
TCCGATC55350.023.8198763
TTCCGAT55950.023.6850722
CCGATCT56300.023.4179424
CTTCCGA56950.023.3680361
CGATCTG24150.022.9180415
CGATCTT12700.021.967345
CTAGCGT750.001111052217.9990464
CGATCTC16800.017.0080265
GTATTAG8800.016.120791
TTAACGG3850.015.19504535
ATAGGGG5350.015.1393833
GTATTAA7950.015.0119531
ATACCCG900.00375553614.99989322
ACCGTCG2405.0931703E-1114.9992058
TACCGTC2253.0559022E-1014.9992057
GATCTAG4400.014.8287596
CGGTACC4100.014.816627545
GGCGTTA2751.8189894E-1214.72716842
GTTTAGG4750.014.6959121
Produced by FastQC (version 0.11.2)