FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell17.351000000951b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell17.351000000951b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141381
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA26610.1242656024313282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23550.023.215795
TCCGATC54100.022.6656063
CCGATCT54450.022.4372714
TTCCGAT54900.022.3763072
CTTCCGA55200.022.2692571
CGATCTA9100.021.0157835
CGATCTT12900.018.8365845
CGGTACC2159.094947E-1216.7432445
GGCGTTA2700.016.66650242
GTATTAG9550.016.5023881
ATCTCGT2057.4578566E-1116.4624827
ATCTGCG2500.016.1990837
GTCCTAT6400.016.1819171
TAATACC4050.016.1105774
TTAACGG2251.8189894E-1116.00096335
TGTTACG850.002566702415.8818262
ACCGTCC1855.631591E-915.8099178
TTAGGAC4450.015.6736363
TCTATCG1754.8661605E-815.429531
CGATCTC16500.015.4085795
Produced by FastQC (version 0.11.2)