FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell16.3510000009519a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell16.3510000009519a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902081
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5750.026.2164425
CTTCCGA36650.024.8241251
TCCGATC36650.024.7398973
TTCCGAT37250.024.5226042
CCGATCT36900.024.4503334
CGATCTG15300.023.6756135
CGATCTT10050.019.2530355
GTATTAG5350.018.9419771
CGTCGGC1551.0913936E-817.41918241
CGATCTC11700.017.1147655
TCTTCCG17800.016.305595
TACCGTC2351.8189894E-1216.2760057
CCGTCGG2151.546141E-1015.69751740
ATAGGGG3300.015.6812513
AATACGC1156.7883375E-515.65160755
AAGTTCG2750.015.545300536
CGGTCAA2950.015.25368418
GCTCTTC19100.015.19578553
GTATTAA6850.015.1228561
CGCGAAG1806.8801455E-814.99985122
Produced by FastQC (version 0.11.2)