FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell15.35100000095170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell15.35100000095170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544739
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG21650.025.4611845
TCCGATC52350.025.1855433
CGATCTA8450.025.0288875
CTTCCGA53750.024.7149521
CCGATCT54100.024.3708534
TTCCGAT54300.024.3225252
CGATCTT15750.020.4285
TCGAGTA1053.1150455E-517.14271541
ATCTGCG3550.017.1118627
CAATCGA800.001711486116.87519338
GCATTCG5200.016.87419722
CGATCTC15050.015.8467335
CGCTAGA5400.015.83351337
GATCTAC5700.015.7890336
GTATTAG8500.015.6285721
TCTTCCG27100.015.608425
GGCGTTA3350.015.447329542
ATTCGTA5750.015.26104424
GTATATA8950.014.8427791
GTCTATA3650.014.804871
Produced by FastQC (version 0.11.2)