FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell14.3510000009514a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell14.3510000009514a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018972
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27810.13774336642608218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC49000.024.4277233
CTTCCGA50100.024.048151
TTCCGAT50250.023.909622
CCGATCT50000.023.8041734
CGATCTG20000.023.736685
CGATCTA8250.022.0901435
CGATCTT11850.021.8346865
GTCGACG750.001111062217.9989343
CGATCTC15800.017.3728165
TATACTG4550.017.3070935
ACGTCCG1205.179938E-616.87399943
CCGTCGA800.001712131316.87399741
CGTCGAC800.001712131316.87399742
TCGACGG800.001712131316.87399744
GATCTGT7900.016.5184156
CGATTGG850.002566235415.88219639
CGGGTAT850.002566659615.88180315
CCGTTAA2600.015.57638316
CGGTACT2900.015.51708938
GTATTAC3050.015.5054751
Produced by FastQC (version 0.11.2)