FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell13.3510000009513d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell13.3510000009513d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688336
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC501.9826311E-631.4994183
CGATCTA5650.030.264935
TCCGATC42150.024.5547073
TTCCGAT42450.024.3811762
CTTCCGA42750.024.2301731
CCGATCT42850.024.153584
CGATCTG18750.022.9195795
TCGTATA500.002265289222.4995842
CGATCTT12450.020.2405915
TACGACG2350.018.1911545
CGACGGT2400.017.8116447
GGCGTTA2053.6379788E-1217.56117242
ACGACGG2500.017.0996866
CGATCTC10700.017.0323965
CGAACTA952.8200969E-416.57913429
GACGGTA2600.016.4415198
GCGTTAT2351.8189894E-1216.27581443
TCTTCCG21400.016.0861515
ATCTAGC2151.546141E-1015.696927
ATCTGCG3050.015.4910597
Produced by FastQC (version 0.11.2)