FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell12.35100000095113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell12.35100000095113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215685
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.16922827271252058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGA250.00210380235.9983343
GCCACTG250.00210380235.9983345
CCTAGTG300.005133436530.0055682
GAAGTGC300.005133436530.0055686
ATTCGGT300.005139262429.9986131
CTATTCG300.005139262429.9986129
CTAGGCC450.00122200225.0046393
GTCGAGT450.001223655924.9988430
GGCAAGG450.001223655924.9988430
AGCTCGC450.001223655924.9988440
GGCCTGC450.001223655924.9988438
GATCTTT25750.023.072246
ATCTTTT25700.022.7615917
AGCACAG500.00225625922.5041735
CTACCCT500.00225625922.5041734
TTTAATA500.00225930322.49895711
TCTTTTT26300.022.2423158
CTTTTTT27450.021.3104889
GGGGTGT654.224187E-420.76826916
ATAAACA654.224187E-420.76826915
Produced by FastQC (version 0.11.2)